Large-Scale Multiple Testing in Medical Informatics

model space experiment space data Joint work with R. virus fruit fly approximately 100 significant genes/protiens discovered by Ahlquist and Kawaoka Labs at UW-Madison virus dep mitochon Treatm mammali cal role o total incu by transfe with FVG post-infec ably affec activity w results ind inhibit ho RNAs and way (see a To test infected si enza A/In VSV or va the mediu (WSN) p COX6A1 decreased (Fig. 3a). (targeting to 13,071 Drosophila genes) to each well of 384-well microplates Add DL1 cells to the plates Infect with FVG-R virus Measure Renilla luciferase activity to assess the efficiency of virus replication 0 h 48 h 72 h Figure 1 | Overview of genome-wide RNAi screen to identify host factors involved in influenza virus replication in Drosophila cells. a, b, Schematic diagrams showing recombinant influenza viruses. Shown are FVG-G, in which genes encoding the HA and NA proteins were replaced with the VSV-G and eGFP genes, respectively (a), and FVG-R, in which the genes encoding the HA and NA were replaced with the VSV-G and Renilla luciferase genes, respectively (b). c, Schematic diagram of the systematic analysis of host genes affecting influenza virus replication and gene expression in Drosophila cells. Experimental details are given in Methods.